Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CNGA2 antibody [C1C3] (GTX112498) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using CNGA2(GTX112498) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded SG xenograft , using CNGA2(GTX112498) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 ug of whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: Hep G2 (GTX27900)
7.5% SDS PAGE
GTX112498 diluted at 1:1000