Wild-type (WT) and COMT knockout (KO) HEK293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with COMT antibody (GTX101233) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ELISA detection of COMT using GTX101233 for capture at a concentration of 2.5 μg/mL and GTX89277 for detection at a concentration of 0.5 μg/mL.

COMT antibody detects COMT protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
COMT antibody (GTX101233) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
12% SDS PAGE
GTX101233 diluted at 1:1000

COMT antibody detects COMT protein by Western blot analysis.
A. 50 μg mouse spleen lysate/extract
12 % SDS-PAGE
COMT antibody (GTX101233) dilution: 1:500