Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N3C3] (GTX114835) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: HCT116
10% SDS PAGE
GTX114835 diluted at 1:1000

The data was published in the journal Nucleic Acids Res in 2013.PMID: 23666625