Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody [GT854] (GTX628901) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

COX4 antibody [GT854] detects COX4I1 protein by western blot analysis.
A. 50 μg rat muscle lysate/extract
15% SDS-PAGE
COX4 antibody [GT854] (GTX628901) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

COX4 antibody [GT854] detects COX4 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
COX4 stained by COX4 antibody [GT854] (GTX628901) diluted at 1:200.
Antigen Retrieval: Tris-EDTA buffer, pH 9.0, 15 min

COX4 antibody detects COX4 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: COX4 stained by COX4 antibody (GTX628901) diluted at 1:1000.

COX4 antibody detects COX4I1 protein by western blot analysis.
A. 50 μg mouse heart lysate/extract
12% SDS-PAGE
COX4 antibody (GTX628901) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.