Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Various whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

CREB antibody [HL2879] detects CREB protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

CREB antibody [HL2879] detects CREB protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat esophagus.
CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CREB antibody [HL2879] detects CREB protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CREB stained by CREB antibody [HL2879] (GTX640158) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CREB antibody [HL2879] (GTX640158) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.