Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CREST antibody [N1C2] (GTX115785) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded Huh-7 xenograft, using CREST(GTX115785) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of CREST protein from MCF-7 whole cell extracts using 5 μg of CREST antibody [N1C2] (GTX115785).
Western blot analysis was performed using CREST antibody [N1C2] (GTX115785).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunofluorescence analysis of paraformaldehyde-fixed A431, using CREST(GTX115785) antibody at 1:500 dilution.