WB analysis of tissue whole cell extracts (30 μg lysate) of Mouse Brain (Lane 1), Rat Brain (lane 2), SH-SY5Y (lane 3), NCCIT (Lane 4), NTERA 2 (Lane 5), and PC 12 (lane 6) using GTX19378 CRMP5 antibody [CR-1].
Dilution : 1:1000

IHC-P analysis of human brain tissue using GTX19378 CRMP5 antibody [CR-1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

ICC/IF analysis of SH-SY5Y cells using GTX19378 CRMP5 antibody [CR-1]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

IHC-P analysis of human glioma tissue using GTX19378 CRMP5 antibody [CR-1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:50