Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CRY1 antibody (GTX132177) diluted at 1:1000.

Treated 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CRY1 antibody (GTX132177) and PER2 antibody (GTX129688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.