Wild-type (WT) and CSK knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CSK antibody (GTX107916) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded HT29 xenograft , using c-Src(GTX107916) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30μg whole cell lysate)
A:293T
B:H1299
C:MOLT4 (GTX27912)
7.5% SDS PAGE
GTX107916 diluted at 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using c-Src(GTX107916) antibody at 1:200 dilution.