Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CSRP1 antibody [N1C3] (GTX114344) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunoprecipitation of CRP1 protein from A549 whole cell extracts using 5 μg of CRP1 antibody [N1C3] (GTX114344).
Western blot analysis was performed using CRP1 antibody [N1C3] (GTX114344) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Sample (30 μg of whole cell lysate)
A: whole zebrafish
12% SDS PAGE
GTX114344 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using CSRP1 antibody [N1C3] (GTX114344) at 1:300 dilution.