Sample (30 ug of whole cell lysate)
A: HUVEC
10% SDS PAGE
GTX124233 diluted at 1:1000

HepG2 conditioned medium (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124233) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CTGF antibody (GTX124233) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.