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CTLA4 antibody [HL2732]

Recombinant antibody
Anti-CTLA4 antibody [HL2732] used in IHC (Paraffin sections) (IHC-P). GTX639560
Anti-CTLA4 antibody [HL2732] used in IHC (Paraffin sections) (IHC-P). GTX639560
Anti-CTLA4 antibody [HL2732] used in Western Blot (WB). GTX639560

Cat. No. GTX639560

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2732

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all CTLA4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant fragment of mouse CTLA4.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cytotoxic T-lymphocyte-associated protein 4 , Cd152 , Ctla-4 , Ly-56

Cellular Localization

Cell membrane

Background

This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-CTLA4 antibody [HL2732] used in IHC (Paraffin sections) (IHC-P). GTX639560

GTX639560 IHC-P Image

CTLA4 antibody [HL2732] detects CTLA4 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
CTLA4 stained by CTLA4 antibody [HL2732] (GTX639560) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CTLA4 antibody [HL2732] used in IHC (Paraffin sections) (IHC-P). GTX639560

GTX639560 IHC-P Image

CTLA4 antibody [HL2732] detects CTLA4 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
CTLA4 stained by CTLA4 antibody [HL2732] (GTX639560) diluted at 1:300.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-CTLA4 antibody [HL2732] used in Western Blot (WB). GTX639560

GTX639560 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CTLA4 antibody [HL2732] (GTX639560) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations