CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm in rat lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
CXCL16 antibody [GT516] (GTX632502) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCL16 antibody [GT516] detects CXCL16 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCL16 stained by CXCL16 antibody [GT516] (GTX632502) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

HeLa whole cell and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

HeLa whole cell extract and membrane extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with CXCL16 antibody [GT516] (GTX632502) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)