CXCR3 antibody [HL2843]

Cat. No. GTX640114

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2843
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX640114 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody was raised against human CXCR3 Extracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Extracellular domain of human CXCR3. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-X-C motif chemokine receptor 3 , CD182 , CD183 , CKR-L2 , CMKAR3 , GPR9 , IP10-R , Mig-R , MigR

Cellular Localization

Cell membrane

Background

This gene encodes a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-g), CXCL10/IP10 (interferon-g-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell a-chemoattractant). Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms (CXCR3-B) shows high affinity binding to chemokine, CXCL4/PF4 (PMID:12782716). [provided by RefSeq, Jun 2011]

Database

Gene ID: 2833 CXCR3
UniProt: P49682 CXCR3

Research Area

DATA IMAGES

GTX640114 ICC/IF Image

CXCR3 antibody [HL2843] detects CXCR3 protein at cell membrane by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR3 stained by CXCR3 antibody [HL2843] (GTX640114) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX640114 WB Image

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR3 antibody [HL2843] (GTX640114) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations