Boiled and unboiled various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3568] (GTX641474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR5 antibody [HL3568] (GTX641474) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.