Non-transfected (–) and transfected (+) SW480 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded Raji xenograft.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:200 and competitor's antibody (MAB42273) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR7 antibody [HL2189] detects CXCR7 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR7 antibody [HL2189] detects CXCR7 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR7 antibody [HL2189] detects CXCR7 protein by immunofluorescent analysis.
Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

CXCR7 antibody [HL2189] detects CXCR7 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Unboiled Raji whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded Raji xenograft.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CXCR7 antibody [HL2189] detects CXCR7 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CXCR7 stained by CXCR7 antibody [HL2189] (GTX638193) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with CXCR7 antibody [HL2189] (GTX638193) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.