Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with CYLD antibody [HL1260] (GTX636662) diluted at 1:1000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CYLD stained by CYLD antibody [HL1260] (GTX636662) diluted at 1:685.
Blue: Fluoroshield with DAPI (GTX30920).

CYLD antibody [HL1260] detects CYLD protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
CYLD stained by CYLD antibody [HL1260] (GTX636662) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min