IHC-P analysis of formalin fixed human brain tissue section using GTX56294 CYP17A1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

WB analysis of HeLa (A), NIH3T3 (B), H9C2 (C), SW13 (D), ES2 (E) whole cell lysates using GTX56294 CYP17A1 antibody.

ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX56294 CYP17A1 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes