Non-transfected (–) and transfected (+) A549 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP24A1 antibody [HL1783] (GTX637435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

CYP24A1 antibody [HL1783] detects CYP24A1 protein at mitochondria by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: CYP24A1 stained by CYP24A1 antibody [HL1783] (GTX637435) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

CYP24A1 antibody [HL1783] detects CYP24A1 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
CYP24A1 stained by CYP24A1 antibody [HL1783] (GTX637435) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CYP24A1 antibody [HL1783] (GTX637435) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP24A1 antibody [HL1783] (GTX637435) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.