Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1819] (GTX637544) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

CYP27A1 antibody [HL1819] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
CYP27A1 stained by CYP27A1 antibody [HL1819] (GTX637544) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CYP27A1 antibody [HL1819] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CYP27A1 stained by CYP27A1 antibody [HL1819] (GTX637544) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min