CYP27A1 antibody [HL1820] detects CYP27A1 protein at mitochondria by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: CYP27A1 stained by CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
CYP27A1 stained by CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

CYP27A1 antibody [HL1820] detects CYP27A1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
CYP27A1 stained by CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CYP27A1 antibody [HL1820] (GTX637545) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.