Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (GTX638587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat cerebellum.
Green: Calbindin stained by Calbindin antibody [HL2379] (GTX638587) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Calbindin antibody [HL2379] detects Calbindin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Green: Calbindin stained by Calbindin antibody [HL2379] (GTX638587) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 , stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Human tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (GTX638587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (GTX638587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calbindin antibody [HL2379] detects Calbindin protein at cytoplasm by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calbindin stained by Calbindin antibody [HL2379] (GTX638587) diluted at 1:250.
Red: Tau, a axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Calbindin antibody [HL2379] (GTX638587) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.