Immunohistochemical analysis of paraffin-embedded SAS xenograft , using Calcium Sensing Receptor(GTX101348) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody (GTX101348) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody (GTX101348) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Calcium Sensing Receptor antibody (GTX101348) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.