Non-transfected (–) and transfected (+) 293T whole cell extracts (15 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody (GTX101676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Sample (30 μg of whole cell lysate)
A:NIH-3T3
7.5% SDS PAGE
GTX101676 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Calnexin antibody [N3C2], Internal detects Calnexin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Calnexin stained by Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Cells in 2019. PMID: 31775322

The data was published in the journal Nat Commun in 2020.PMID: 32111843