WB analysis of various samples using GTX00742 Calnexin antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Dilution : 1:1000
Loading : 20 μg

WB analysis of mouse testis or sperm tissue lysate using GTX00742 Calnexin antibody.
Dilution : 1:1000
Loading : 10 μg

WB analysis of mouse testis extracts of different ages (1-6 weeks) using GTX00742 Calnexin antibody.
Dilution : 1:1000
Loading : 20 μg

IHC-P analysis of mouse testis tissue section using GTX00742 Calnexin antibody.
Dilution : 1:100
Antigen retrieval : Proteolytic-Induced Epitope Retrieval

IP analysis of mouse testis using GTX00742 Calnexin antibody.
Lane 1 : Input mouse testis lysate
Lane 2 : Precipitated with preimmune serum
Lane 3 : Precipitated with anti-CANX antibody
Total protein amount : 1 mg
IP antibody : 2 μg
IB antibody : 1:1000
Total protein amount : 1 mg

ICC/IF analysis of HeLa cells using GTX00742 Calnexin antibody.
Dilution : 1:100 or 1:300
Fixation : 4 % PFA at RT for 30 min or at 4 degree C for, overnight
Permeabilization : 0.25 % Triton X-100 in PBS for 10 min