WB analysis of various mouse tissue samples using GTX00741 Calreticulin 3 antibody.
Lane 1 : mouse brain tissue lysate
Lane 2 : mouse lung tissue lysate
Lane 3 : mouse heart tissue lysate
Lane 4 : mouse thymus tissue lysate
Lane 5 : mouse liver tissue lysate
Lane 6 : mouse spleen tissue lysate
Lane 7 : mouse kidney tissue lysate
Lane 8 : mouse muscle tissue lysate
Lane 9 : mouse ovary tissue lysate
Lane 10 : mouse testis tissue lysate
Lane 11 : mouse sperm tissue lysate
Dilution : 1:1000

WB analysis of mouse testis or sperm tissue lysate using GTX00741 Calreticulin 3 antibody. This protein is testis specific and absent in mature sperm.
Dilution : 1:1000
Loading : 10 μg
SDS-PAGE : 10-20% gradient gel

IHC-Fr analysis of mouse testicular tissue section (8 μm) using GTX00741 Calreticulin 3 antibody.
Upper : hematoxylin and eosin staining
Lower : Primary antibody
Dilution : 1:100
Fixation : 4% PFA in PBS, overnight, 4 degree C
Scale bar : 200 μm

WB analysis of embryonic fibroblast cells prepared from Calr3 -/- mouse using GTX00741 Calreticulin 3 antibody.
Lane 1 : Mock
Lane 2 : Cells overexpressed Calr3 protein
Dilution : 1:1000

IP analysis of wild-type mouse testis using GTX00741 Calreticulin 3 antibody.
Lane 1 : Input mouse testis lysate
Lane 2 : Precipitated with preimmune serum
Lane 3 : Precipitated with anti-CALR antibody