Reactivity of GTX00867 Campylobacter Porin antibody [cj-01] with Campylobacter jejuni in immunochromatographic strip test. Rabbit anti-C. jejuni and goat anti-Salmonella Enteritidis sera containing IgG, IgA and IgM were coated onto a specific areas of the samenitrocellulose membrane as shown, while goat anti-mouse IgG was coated onto another specific area (control line) on the same membrane. GTX00867 or S. Enteritidis monoclonal antibody conjugated with colloidal gold was mixed with extract of each strain of food poisoning bacteria in well. The strips were soaked in and reacted with the mixture fluid in the wells. Specific bands were observed in the strips reacted with extract of S. Enteritideis (left) or C. jujeni (right).

Specific reactivity of GTX00867 Campylobacter Porin antibody [cj-01] with various food poisoning bacteria by ELISA and WB.

ELISA analysis of crude extract of Campylobacter jejuni using GTX00867 Campylobacter Porin antibody [cj-01].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

Test of reactivity of GTX00867 Campylobacter Porin antibody [cj-01] with several food poisoning bacteria in slot blot test. Extract of each strain of food poisoning bacteria was coated onto 5 areas of a nitrocellulose membrane and reacted with GTX00867. GTX00867 specifically reacts with extract of C. jejuni.
SE: Salmonella Enteritidis
EC: Escherichia coli
CJ: C. jejuni
VP: Vibrio parahaemolyticus

WB analysis of various samples using GTX00867 Campylobacter Porin antibody [cj-01]. It recognizes porins in extracts of C. coli and C. jejuni as 44 kDa protein and 43 kDa protein, respectively.
Lane 1 : Crude extract of Campylobacter coli
Lane 2 : Crude extract of Campylobacter jejuni