Non-transfected (–) and transfected (+) unboiled Jurkat whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Cannabinoid receptor 2 antibody [HL3120] detects Cannabinoid receptor 2 protein by immunofluorescent analysis.
Sample: Jurkat cells were fixed in ice-cold MeOH for 5 min.
Green: Cannabinoid receptor 2 stained by Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Boiled and unboiled Jurkat whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cannabinoid receptor 2 antibody [HL3120] (GTX640596) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.