Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Carbonic Anhydrase IX antibody [HL1410] detects Carbonic Anhydrase IX protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Carbonic Anhydrase IX stained by Carbonic Anhydrase IX antibody [HL1410] (GTX636876) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10μm.