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Carbonic Anhydrase IX antibody

Overexpression
Anti-Carbonic Anhydrase IX antibody used in IHC (Paraffin sections) (IHC-P). GTX15086
Anti-Carbonic Anhydrase IX antibody used in IHC (Paraffin sections) (IHC-P). GTX15086
Anti-Carbonic Anhydrase IX antibody used in Western Blot (WB). GTX15086
Anti-Carbonic Anhydrase IX antibody used in Western Blot (WB). GTX15086
Anti-Carbonic Anhydrase IX antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15086
Anti-Carbonic Anhydrase IX antibody used in Western Blot (WB). GTX15086
Anti-Carbonic Anhydrase IX antibody used in Western Blot (WB). GTX15086
Anti-Carbonic Anhydrase IX antibody used in IHC (Frozen sections) (IHC-Fr). GTX15086

Cat. No. GTX15086

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, ELISA, ChIP assay, Gel supershift assays

Reactivity

 Human, Mouse, Rat, Dog
Package
100 μl ($449)
See all Carbonic Anhydrase IX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:1000
IHC-P 1:1000
IHC-Fr 1:1000
FACS Assay dependent
ELISA Assay dependent
ChIP assay 1:10 - 1:500
Gel supershift assays Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide made to a C-terminal sequence of human Carbonic Anhydrase IX (within residues 400-459) [UniProt Q16790]

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_368767

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

carbonic anhydrase 9 , CAIX , MN

Cellular Localization

Nucleus,Cell membrane,Nucleolus

Background

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation. This gene was mapped to 17q21.2 by fluorescence in situ hybridization, however, radiation hybrid mapping localized it to 9p13-p12. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Carbonic Anhydrase IX antibody used in IHC (Paraffin sections) (IHC-P). GTX15086

GTX15086 IHC-P Image

IHC-P analysis of renal carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody.

Anti-Carbonic Anhydrase IX antibody used in IHC (Paraffin sections) (IHC-P). GTX15086

GTX15086 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody.
Dilution : 1:1000

Anti-Carbonic Anhydrase IX antibody used in Western Blot (WB). GTX15086

GTX15086 WB Image

Overexpression

WB analysis of HEK overexpressing Carbonic Anhydrase IX cell lysate using GTX15086 Carbonic Anhydrase IX antibody. Rabbit IgG was used as a negative control.

Anti-Carbonic Anhydrase IX antibody used in Western Blot (WB). GTX15086

GTX15086 WB Image

WB analysis of rat renal cortex tissue lysate using GTX15086 Carbonic Anhydrase IX antibody.

Anti-Carbonic Anhydrase IX antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15086

GTX15086 ICC/IF Image

Overexpression

ICC/IF analysis of HEK 293 cells overexpressing Carbonic Anhydrase IX (Panel A) and mock cells (Panel B) using GTX15086 Carbonic Anhydrase IX antibody.

Anti-Carbonic Anhydrase IX antibody used in Western Blot (WB). GTX15086

GTX15086 WB Image

WB analysis of (1)HeLa, (2)MDA-MB-231 and (3)A549 cell lysate using GTX15086 Carbonic Anhydrase IX antibody.

Anti-Carbonic Anhydrase IX antibody used in Western Blot (WB). GTX15086

GTX15086 WB Image

WB analysis of HeLa cell lysate using GTX15086 Carbonic Anhydrase IX antibody.

Anti-Carbonic Anhydrase IX antibody used in IHC (Frozen sections) (IHC-Fr). GTX15086

GTX15086 IHC-Fr Image

IHC-Fr analysis of renal carcinoma tissue using GTX15086 Carbonic Anhydrase IX antibody.
Panel A : primary antibody
Panel B : staining with normal rabbit serum

REFERENCE

Application Reference

Veelken C et al. Methods 2017; (Epub) Single cell-based automated quantification of therapy responses of invasive cancer spheroids in organotypic 3D culture.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449