Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Indirect ELISA analysis was performed by coating the plate with recombinant E.coli expressed, full-length Human Cardiac Troponin I protein, His tag (GTX109331-pro) (41.65-0.65 nM). Coated protein was probed with Cardiac Troponin I antibody [HL1489] (GTX636963) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect the bound primary antibody.

Human tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat heart.
Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cardiac Troponin I antibody [HL1489] detects Cardiac Troponin I protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Cardiac Troponin I stained by Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Cardiac Troponin I antibody [HL1489] (GTX636963) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.