Caspase 1 antibody [N1N3] detects Caspase 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Caspase 1 stained by Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha(GTX101322) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 alpha(GTX101322) antibody at 1:500 dilution.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23653660

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23653660