Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody (GTX111630) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Caspase 1(GTX111630) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 beta(GTX111630) antibody at 1:500 dilution.

The data was published in the journal Front Cell Infect Microbiol in 2018.PMID: 30186771