Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Wild-type (WT) and Caspase 3 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Caspase 3 (cleaved Asp175) antibody [HL1370] (GTX636810) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Caspase 3 antibody [HL1370] detects Caspase 3 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human tonsil.
Caspase 3 stained by Caspase 3 antibody [HL1370] (GTX636810) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min