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Caspase 9 antibody [LAP6 96-2-22]

Anti-Caspase 9 antibody [LAP6 96-2-22] used in Western Blot (WB). GTX30245
Anti-Caspase 9 antibody [LAP6 96-2-22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30245
Anti-Caspase 9 antibody [LAP6 96-2-22] used in Western Blot (WB). GTX30245

Cat. No. GTX30245

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 LAP6 96-2-22

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
200 μl ($419)
See all Caspase 9 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:50 - 1:250
IHC-P 1:50 - 1:250
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant human Caspase 9 pro-domain protein. [UniProt# P55211]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 9 , APAF-3 , APAF3 , ICE-LAP6 , MCH6 , PPP1R56

Background

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Caspase 9 antibody [LAP6 96-2-22] used in Western Blot (WB). GTX30245

GTX30245 WB Image

WB analysis of active 293 (lane 2) and inactive 293 cell lysate(lane 1) using GTX30245 Caspase 9 antibody [LAP6 96-2-22].

Anti-Caspase 9 antibody [LAP6 96-2-22] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30245

GTX30245 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX30245 Caspase 9 antibody [LAP6 96-2-22].
Green : primary antibody
Red : Tubulin
Blue : DAPI

Anti-Caspase 9 antibody [LAP6 96-2-22] used in Western Blot (WB). GTX30245

GTX30245 WB Image

WB analysis of Jurkat (A) and HeLa (B) cell lysate using GTX30245 Caspase 9 antibody [LAP6 96-2-22].
Dilution : 1 : 500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 419