FACS analysis of L6 cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of cells using GTX79350 Caveolin 1 antibody [7C8].

ICC/IF analysis of HeLa cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 in PBS for 15 minutes
Dilution : 1:100 for 1 hour at room temperature

IHC-P analysis of rat lymph node tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

WB analysis of 25 ug of A431 (Lane 1) and rat lung (Lane 2) cell lysates using GTX79350 Caveolin 1 antibody [7C8].
Dilution : 1:1000

IHC-P analysis of rat lung tissue using GTX79350 Caveolin 1 antibody [7C8].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

FACS analysis of H-4-II-E cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde

ICC/IF analysis of H-4-II-E Cells using GTX79350 Caveolin 1 antibody [7C8]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

FACS analysis of CHO cells using GTX79350 Caveolin 1 antibody [7C8] compared to an isotype control (blue).
Dilution : 1:20 for 60 min at room temperature
Fixation : 2% paraformaldehyde