Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HCT116 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Chk1 (phosphpo Ser345) antibody [HL122] detects Chk1 (phosphpo Ser345) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Chk1 (phosphpo Ser345) stained by Chk1 (phosphpo Ser345) antibody [HL122] (GTX635572) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).