Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
Citrate synthetase antibody [N2C3] (GTX110624) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Citrate synthetase antibody detects Citrate synthetase protein by Western blot analysis. Rat tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with Citrate synthetase antibody (GTX110624) at a dilution of 1:1000.

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat heart.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at cytoplasm in mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of Citrate synthetase protein from 293T whole cell extracts using 5 μg of Citrate synthetase antibody [N2C3] (GTX110624).
Western blot analysis was performed using Citrate synthetase antibody [N2C3] (GTX110624).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Cs antibody (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Citrate synthase antibody [N2C3] detects Citrate synthase protein on whole mount zebrafish by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 1 day-post-fertilization zebrafish embryo.
Green: Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Citrate synthase antibody [N2C3] detects Citrate synthase protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Citrate synthase antibody [N2C3] (GTX110624) dilution: 1:50.

Citrate synthetase antibody [N2C3] detects Citrate synthetase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Citrate synthetase stained by Citrate synthetase antibody [N2C3] (GTX110624) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Citrate synthase antibody [N2C3] detects Citrate synthase protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Citrate synthase stained by Citrate synthase antibody [N2C3] (GTX110624) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

The data was published in the journal Sci Rep in 2017. PMID: 28515438

The data was published in the journal Sci Rep in 2017. PMID: 28515438

The data was published in the journal PLoS One in 2014. PMID: 25489948