Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 3 antibody [HL1527] (GTX637000) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunofluorescent analysis.
Sample: MCF-7 and HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Claudin 3 stained by Claudin 3 antibody [HL1527] (GTX637000) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Claudin 3 antibody [HL1527] detects Claudin 3 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Claudin 3 stained by Claudin 3 antibody [HL1527] (GTX637000) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min