Unboiled mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Unboiled various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.