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Claudin 5 antibody [HL3156]

Recombinant antibody Orthogonal
Anti-Claudin 5 antibody [HL3156] used in Western Blot (WB). GTX640666
Anti-Claudin 5 antibody [HL3156] used in Western Blot (WB). GTX640666

Cat. No. GTX640666

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL3156

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Claudin 5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human Claudin 5.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

claudin 5 , AWAL , BEC1 , CPETRL1 , TMDVCF , TMVCF

Cellular Localization

Cell junction, tight junction,Cell membrane; Multi-pass membrane protein

Background

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, May 2018]

Database

Research Area

DATA IMAGES

Anti-Claudin 5 antibody [HL3156] used in Western Blot (WB). GTX640666

GTX640666 WB Image

Orthogonal

Unboiled various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Claudin 5 antibody [HL3156] used in Western Blot (WB). GTX640666

GTX640666 WB Image

Orthogonal

Unboiled mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 5 antibody [HL3156] (GTX640666) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations