HeLa whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Coilin antibody (GTX112570) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Coilin antibody detects Coilin protein by Western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg 293T nuclear extract
7.5 % SDS-PAGE
Coilin antibody (GTX112570) dilution: 1:1000

Coilin antibody detects Coilin protein by Western blot analysis.
A. 30 μg HepG2 whole cell extract
B. 30 μg HepG2 nuclear extract
7.5 % SDS-PAGE
Coilin antibody (GTX112570) dilution: 1:1000

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Coilin(GTX112570) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

Coilin antibody detects Coilin protein at nucleus in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
Coilin antibody (GTX112570) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 μg of Coilin antibody (GTX112570).
Western blot analysis was performed using Coilin antibody (GTX112570).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.