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Collagen I antibody

Orthogonal
Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292
Anti-Collagen I antibody used in Western Blot (WB). GTX20292
Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292
Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292
Anti-Collagen I antibody used in Western Blot (WB). GTX20292
Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292
Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292
Anti-Collagen I antibody used in Western Blot (WB). GTX20292
Anti-Collagen I antibody used in Western Blot (WB). GTX20292

Cat. No. GTX20292

Reference 13

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, IP, Dot, ELISA, Multiplexing

Reactivity

 Human, Mouse, Rat, Bovine, Hamster, Pig
Package
100 μg ($399)
See all Collagen I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:50-1:200
FACS Assay dependent
IP 1:100
Dot Assay dependent
ELISA 1:5000-1:50000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Product Note

Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human, Bovine

Immunogen

Collagen Type I from human and bovine placenta

Purification

Purified by antigen-affinity chromatography.
This product was prepared by immunoaffinity chromatography on immobilized antigens followed by extensive cross-adsorption against other collagens, human serum proteins and non-collagen extracellular matrix proteins to remove unwanted specificities.

Conjugation

Unconjugated

RRID

AB_384293

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

collagen type I alpha 1 chain , EDSARTH1 , EDSC , OI1 , OI2 , OI3 , OI4

Cellular Localization

Secreted,Extracellular space,Extracellular matrix

Background

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted ""Glycine-X-Y"" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. This preparation results in a native conformation of the protein.

Database

Research Area

DATA IMAGES

Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292

GTX20292 IHC-P Image

IHC-P analysis of human lung tissue section using GTX20292 Collagen I antibody.
Antigen retrieval : user optimized
Dilution : 1:400
Localization : Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative.
Staining : antibody as precipitated red signal with a hematoxylin purple nuclear counterstain.

Anti-Collagen I antibody used in Western Blot (WB). GTX20292

GTX20292 WB Image

Western blot of Human Collagen Type I. Load: 50 ng Human Collagen Type 1. Primary antibody: Collagen Type I antibody (GTX20292) at 1:1,000 overnight at 4ºC. Secondary antibody: DyLight™ 649 rabbit secondary antibody at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 139 & 130 kDa.

Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292

GTX20292 IHC-P Image

IHC-P analysis of normal kidney tissue section using GTX20292 Collagen I antibody.
Antigen retrieval : No antigen retrieval was performed.
Dilution : 1:100
Localization : Distal tubules in normal kidney tissue.
Note : the absence of staining of glomeruli.

Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292

GTX20292 IHC-P Image

IHC-P analysis of liver tissue using GTX20292 Collagen I antibody.
Panel A : Central Vein (CV) fibrosis
Panel B : Non-fibrotic Central Vein (CV)
Panel C : Perisinusodial fibrosis
Panel D : Non-fibrotic area
Panel E : Protat tract fibrosis
Panel F : Septal fibrosis (arrow)
Antigen retrieval: not performed.
Dilution : 1:1250

Anti-Collagen I antibody used in Western Blot (WB). GTX20292

GTX20292 WB Image

WB analysis of human Collagen Type I protein using GTX20292 Collagen I antibody.
Loading : 50 ng
Dilution : 1:1000

Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292

GTX20292 IHC-P Image

IHC-P analysis of human lung tissue using GTX20292 Collagen I antibody.
Strong staining was observed in the extracellular matrix of the lung. Epithelial cells were negative.
Dilution : 1:400

Anti-Collagen I antibody used in IHC (Paraffin sections) (IHC-P). GTX20292

GTX20292 IHC-P Image

IHC-P analysis of normal mouse kidney tissue using GTX20292 Collagen I antibody.
Antigen retrieval: not performed.
Dilution : 1:100

Anti-Collagen I antibody used in Western Blot (WB). GTX20292

GTX20292 WB Image

Orthogonal

The data was published in the journal J Cell Mol Med in 2019.PMID: 30411499

Anti-Collagen I antibody used in Western Blot (WB). GTX20292

GTX20292 WB Image

The data was published in the journal Front Pharmacol in 2016. PMID: 27199755

REFERENCE

Application Reference

Li YJ et al. iScience 2023; 26 (5) : 106586 Phosphodiesterase type 10A inhibitor attenuates lung fibrosis by targeting myofibroblast activation.
Chen CS et al. BMC Complement Med Ther 2022; 22 (1) : 271 Fallopia Japonica and Prunella vulgaris inhibit myopia progression by suppressing AKT and NFκB mediated inflammatory reactions.
Ku H et al. Curr Issues Mol Biol 2022; 44 (9) : 4303-4313 Myopia Development in Tree Shrew Is Associated with Chronic Inflammatory Reactions.
Yunmee Lho et al. Int J Mol Sci 2021; 22 (9) Effects of TGF-β1 Receptor Inhibitor GW788388 on the Epithelial to Mesenchymal Transition of Peritoneal Mesothelial Cells.
Application : ICC/IF, IHC-P
Reactivity : Mouse
Tien PT et al. Mediators Inflamm 2021; 2021 : 6660640 Diacerein Inhibits Myopia Progression through Lowering Inflammation in Retinal Pigment Epithelial Cell.
Application : IHC-P
Reactivity : Hamster
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REVIEW

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Application Reference
Li YJ et al. iScience 2023; 26 (5):106586 Phosphodiesterase type 10A inhibitor attenuates lung fibrosis by targeting myofibroblast activation.
Chen CS et al. BMC Complement Med Ther 2022; 22 (1):271 Fallopia Japonica and Prunella vulgaris inhibit myopia progression by suppressing AKT and NFκB mediated inflammatory reactions.
Ku H et al. Curr Issues Mol Biol 2022; 44 (9):4303-4313 Myopia Development in Tree Shrew Is Associated with Chronic Inflammatory Reactions.
Yunmee Lho et al. Int J Mol Sci 2021; 22 (9) Effects of TGF-β1 Receptor Inhibitor GW788388 on the Epithelial to Mesenchymal Transition of Peritoneal Mesothelial Cells.
Application : ICC/IF, IHC-P
Reactivity : Mouse
Heo JY et al. Biomedicines 2021; 9 (7) TGF-β1 Receptor Inhibitor SB525334 Attenuates the Epithelial to Mesenchymal Transition of Peritoneal Mesothelial Cells via the TGF-β1 Signaling Pathway.
Application : IHC-P
Reactivity : Human
Tien PT et al. Mediators Inflamm 2021; 2021:6660640 Diacerein Inhibits Myopia Progression through Lowering Inflammation in Retinal Pigment Epithelial Cell.
Application : IHC-P
Reactivity : Hamster
Wong K et al. Drug Des Devel Ther 2018; 12:15-24 Intra-articular collagenase injection increases range of motion in a rat knee flexion contracture model.
Wei CC et al. EBioMedicine 2018; 28:274-286 Allergic Conjunctivitis-induced Retinal Inflammation Promotes Myopia Progression.
Zhang M et al. J Cell Mol Med 2018; (Epub) Bilateral sympathetic stellate ganglionectomy attenuates myocardial remodelling and fibrosis in a rat model of chronic volume overload.
Application : WB
Reactivity : Rat
Schendel SA et al. Aesthet Surg J 2017; (Epub) Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.
Application : WB
Reactivity : Human
Chen MF et al. BMC Cancer 2016; 16:532 Saikosaponin d induces cell death through caspase-3-dependent, caspase-3-independent and mitochondrial pathways in mammalian hepatic stellate cells.
Application : WB
Reactivity : Human
Chuang HM et al. Front Pharmacol 2016; 7:112 The Role of Butylidenephthalide in Targeting the Microenvironment Which Contributes to Liver Fibrosis Amelioration.
Application : WB
Reactivity : Rat
Choi JY et al. Biochem Biophys Res Commun 2015; 468 (4):883-8 Lyso-globotriaosylceramide downregulates KCa3.1 channel expression to inhibit collagen synthesis in fibroblasts.
Application : WB
Reactivity : Mouse
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399