Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III antibody [C2C3-2], C-term (GTX111643) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using Collagen III (GTX111643) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Collagen III antibody [C2C3-2], C-term detects Collagen III protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Collagen III stained by Collagen III antibody [C2C3-2], C-term (GTX111643) diluted at 1:500.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Collagen III alpha1 antibody [C2C3-2], C-term (GTX111643) diluted at 1:1000.

The data was published in the journal Sci Rep in 2017. PMID: 29170419