Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1:500 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Using immunohistochemical staining of basal laminae, the antibody recognizes an epitope located on the α1 and/or α2 chains of human collagen type IV. The antibody shows reactivity with native primate collagen type IV in ELISA and dot blot assay. Using the dot blot technique, the antibody shows no cross-reactivity with human collagen types I, II, III, V, VI and VII, human vitronectin, fibronectin and chondroitin sulfate types A, B and C.
We do not recommend use of this product for Rat,Rabbit,Goat,Sheep,Cat,Chicken samples.
Form
Liquid
Buffer
Ascites
Preservative
15mM Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
human collagen type IV
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_368873
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
collagen type IV alpha 1 chain , BSVD , RATOR
Background
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio . It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
Database
Research Area