Connexin 43 antibody detects Connexin 43 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Connexin 43 antibody detects Connexin 43 protein at cell membrane by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Connexin 43 antibody (GTX133022) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) SK-N-SH whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Connexin 43 antibody (GTX133022) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Connexin 43 antibody detects Connexin 43 protein by immunofluorescent analysis.
Sample: U87-MG cells were fixed in ice-cold MeOH for 5 min.
Green: Connexin 43 stained by Connexin 43 antibody (GTX133022) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker diluted at 1:200.
Blue: Hoechst 33342 staining.