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Connexin 43 antibody

Anti-Connexin 43 antibody used in Western Blot (WB). GTX54818
Anti-Connexin 43 antibody used in Western Blot (WB). GTX54818
Anti-Connexin 43 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX54818
Anti-Connexin 43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54818

Cat. No. GTX54818

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all Connexin 43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

43 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 (Intracellular, C-terminus) of human Connexin-43 (Accession  : P17302).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

gap junction protein alpha 1 , AVSD3 , CMDR , CX43 , EKVP , EKVP3 , GJAL , HLHS1 , HSS , ODDD , PPKCA

Cellular Localization

Cell membrane,Endoplasmic reticulum

Background

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. The encoded protein is the major protein of gap junctions in the heart that are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. A related intronless pseudogene has been mapped to chromosome 5. Mutations in this gene have been associated with oculodentodigital dysplasia, autosomal recessive craniometaphyseal dysplasia and heart malformations. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-Connexin 43 antibody used in Western Blot (WB). GTX54818

GTX54818 WB Image

WB analysis of rat heart membrane lysate using GTX54818 Connexin 43 antibody preincubated with or without immunogen peptide.
Dilution : 1:400

Anti-Connexin 43 antibody used in Western Blot (WB). GTX54818

GTX54818 WB Image

IHC-Frfl (free floating) analysis of rat cerebellum tissue using GTX54818 Connexin 43 antibody. Cx43 staining (red) appeared in Bergmann glial fibers (arrows) in the molecular layer (MOL) and in the granule layer (G). Cell nuclei were stained with DAPI (blue).
Dilution : 1:300

Anti-Connexin 43 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX54818

GTX54818 IHC (Free Floating) Image

WB analysis of mouse brain membrane lysates using GTX54818 Connexin 43 antibody preincubated with or without immunogen peptide.
Dilution : 1:400

Anti-Connexin 43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54818

GTX54818 ICC/IF Image

ICC/IF analysis of IEC-6  cells using GTX54818 Connexin 43 antibody.
Red : Primary antibody
Blue : DNA dye Hoechst 33342
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639