Wild-type (WT) and Coronin 1C knockout (KO) HEK293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded human Sarcoma meta to brain Blood Vessel, using CORO1C (GTX106469) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using Coronin 1C(GTX106469) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Coronin 1C antibody [C3], C-term (GTX106469) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.