APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1-2μg/ml |
Note :
IHC-P
Mouse Embryonic Liver shows staining of the hepatocyte cell membranes.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89499-PEP).
Predict Reactivity
Human, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence HFTGRYCEHDQRRSEC, from the internal region of the protein sequence according to NP_115934.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
cripto, FRL-1, cryptic family 1 , CFC1B , CRYPTIC , DTGA2 , HTX2
Cellular Localization
Cell membrane , Secreted
Background
This gene encodes a member of the epidermal growth factor (EGF)- Cripto, Frl-1, and Cryptic (CFC) family, which are involved in signalling during embryonic development. Proteins in this family share a variant EGF-like motif, a conserved cysteine-rich domain, and a C-terminal hydrophobic region. The protein encoded by this gene is necessary for patterning the left-right embryonic axis. Mutations in this gene are associated with defects in organ development, including autosomal visceral heterotaxy and congenital heart disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
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GTX89499 IHC-P Image
IHC-P analysis of mouse embryo liver using GTX89499 Cryptic antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 2μg/ml
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REFERENCE
There are currently no references for Cryptic antibody, Internal (GTX89499). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Cryptic antibody, Internal (GTX89499). Be the first to share your experience with this product.