Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Cullin 7 antibody [N2N3] (GTX109785) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (30 ug of whole cell lysate)
A: Raji
5% SDS PAGE
GTX109785 diluted at 1:2000