Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated)
B. 30 μg HeLa whole cell lysate/extract (serum starvation for 48hr)
C. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 3.5hr)
D. 30 μg HeLa whole cell lysate/extract (2mM thymidine treatment and recovery for 12hr)
10 % SDS-PAGE
Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:500

Cyclin B1 antibody [GT238] detects CCNB1 protein by Western blot analysis.
A. 30 μg U2OS whole cell lysate/extract (untreated)
B. 30 μg U2OS whole cell lysate/extract (100ng/ml Nocodazole treatment for 24hr)
10 % SDS-PAGE
Cyclin B1 antibody [GT238] (GTX628546) dilution: 1:1000

Cyclin B1 antibody [GT238] detects Cyclin B1 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Cyclin B1 stained by Cyclin B1 antibody [GT238] (GTX628546) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min